TABLE 1 .
Results showing the presence/absence of phopivirus in different animals and tissues by both qPCR and conventional PCR
| Animala | Result of qPCR (conventional PCR) inb: |
|||||||
|---|---|---|---|---|---|---|---|---|
| Adrenal gland | Dermal lesion | Liver | Lung | Oral mucosa | Spleen | Thoracic lymph node | Skin | |
| COA120801 Pv | Neg (neg) | |||||||
| IFAW11 279 Pv | Neg (neg) | |||||||
| IFAW11 280 Pv | Neg (neg) | |||||||
| IFAW11 283 Pv | Neg (neg) | |||||||
| IFAW12 258 Pv | Neg (neg) | |||||||
| MARC 12 046 Pv | Neg (neg) | |||||||
| MARC 12 052 Pv | Neg (neg) | |||||||
| MH 08 035 Pg | Neg (neg) | Neg (neg) | Neg (neg) | |||||
| MH 08 048 Pv | Neg (neg) | Neg (neg) | Neg (neg) | |||||
| NEAQ 11 006 Pgc | Pos (neg) | Pos (neg) | Pos (pos) | |||||
| NEAQ 11 175 Pv | Neg (neg) | Neg (neg) | Neg (neg) | |||||
| NEAQ 11 239 Pv | Neg (neg) | Neg (neg) | Neg (neg) | |||||
| NEAQ 11 250 Pv | Neg (neg) | |||||||
| NEAQ 11 254 Pv | Neg (neg) | |||||||
| NEAQ 11 263 Pv | Neg (neg) | Neg (neg) | Neg (neg) | |||||
| NEAQ 11 278 Pv | Pos (neg) | Neg (neg) | Pos (neg) | |||||
| NEAQ 11 280 Pv | Neg (neg) | Pos (neg) | Pos (neg) | |||||
| NEAQ 11 286 Pvd | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | ||
| NEAQ 11 294 Pvd | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | ||
| NEAQ 11 295 Pvd | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | ||
| NEAQ 11 300 Pv | Neg (neg) | Neg (neg) | Neg | |||||
| NEAQ 11 303 Pv | Pos (pos) | Pos (pos) | Pos (pos) | |||||
| NEAQ 11 306 Pv | Pos (pos) | Pos (pos) | ||||||
| NEAQ 11 318 Pv | Pos (pos) | Pos (pos) | Pos (neg) | Pos (pos) | ||||
| NEAQ 11 352 Pv | Neg (neg) | Neg (neg) | Neg (neg) | Neg (neg) | ||||
| NEAQ 11 354 Pv | Pos (pos) | Pos (neg) | Pos (neg) | Pos (neg) | ||||
| NEAQ 11 355 Pg | Neg (neg) | Neg (neg) | Neg (neg) | |||||
| NEAQ 12 015 Pg | Neg (neg) | Neg (neg) | Neg (neg) | |||||
| NEAQ 12 045 Hg | Neg (neg) | Neg (neg) | Neg (neg) | Neg (neg) | ||||
| NEAQ 12 068 Hg | Neg (neg) | Neg (neg) | Neg (neg) | |||||
| NEAQ 12 156 Pv | Neg (neg) | Neg (neg) | Neg (neg) | Neg (neg) | ||||
| NEAQ 12 159 Pv | Neg (neg) | Neg (neg) | Neg (neg) | Neg (neg) | ||||
| NEAQ 12 180 Pv | Pos (pos) | Pos (pos) | Pos (pos) | |||||
| NEAQ 12 199 Pv | Pos (pos) | Pos (pos) | Pos (pos) | Pos (pos) | ||||
| NEAQ 13 022 Pv | Neg (neg) | Neg (neg) | Neg (neg) | Neg (neg) | Neg (neg) | |||
| NEAQ 13 039 Pg | Neg (neg) | Neg (neg) | Neg (neg) | Neg (neg) | ||||
| NEAQ 13 070 Pg | Neg (neg) | Neg (neg) | Neg (neg) | Neg (neg) | ||||
a
Pg, Phoca groenlandicus (harp seal); Pv, Phoca vitulina (harbor seal); Hg, Halichoerus grypus (gray seal).
b
Results for samples with a positive qPCR are shown in bold font. Pos, positive; Neg, negative.
c
The sequence found in this animal is slightly different (97% identical) from the one found in harbor seals.
d
Samples from the indicated animals were used for fluorescent in situ hybridization (FISH).