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. 2015 Aug 25;6(4):e01206-15. doi: 10.1128/mBio.01206-15

FIG 2 .

FIG 2 

Expression of HMW1CKk is required for adherence and autoaggregation. (A) Mean adherence of K. kingae with the wild-type hmw1CKk gene, an interrupted hmw1CKk gene, and the interrupted hmw1CKk gene reverted to the wild type. (B) Mean autoaggregation of K. kingae. The pilA1 mutant lacks type IV pili, the ctrA mutant lacks the polysaccharide capsule, the knh mutant lacks the trimeric autotransporter protein Knh, and the hmw1CKk mutant lacks HMW1CKk. Graphs in panels A and B show data from biological replicates performed in triplicate. Error bars represent standard errors. An asterisk denotes a P value of <0.05 obtained from a paired t test. (C) Western blot of the formic acid-treated outer membrane fraction of wild-type K. kingae (lane 1), K. kingae with an interrupted hmw1CKk gene (lane 2), and K. kingae with the interrupted hmw1CKk gene reverted to the wild type (lane 3) using an antiserum raised against the Knh protein. (D) Qualitative adherence assay using E. coli strain BL21 expressing HMW1CKk (frame 1), Knh (frame 2), or HMW1CKk and Knh (frame 3).