TABLE 4 .
Bacterial strains
| Strain | Description | Reference |
|---|---|---|
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE441 thi-1 gyrA96 relA | |
| 269–492 (KK03) | Clinical isolate, spontaneous spreading, corroding colony variant of 269-492 | 16 |
| KK03 hmw1CKk::kan | Interruption of hmw1CKk with kanamycin resistance cassette | This study |
| KK03 hmw1CKk::kan(hmw1CKk) | Reversion of interrupted hmw1CKk to wild type | This study |
| KK03 crtA::erm | Interruption of ctrA with erythromycin resistance cassette | This study |
| KK03 pilA1::cm | Interruption of pilA1 with chloramphenicol resistance cassette | This study |
| KK03 ctrA::erm pilA1::cm | Interruption of ctrA with erythromycin resistance cassette and pilA1 with chloramphenicol resistance cassette | This study |
| KK03 ctrA::erm pilA1::cm hmw1CKk::kan | Interruption of ctrA with erythromycin resistance cassette, pilA1 with chloramphenicol resistance cassette, and hmw1CKk with kanamycin resistance cassette | This study |
| KK03 ctrA::erm pilA1::cm knh::kan | Interruption of ctrA with erythromycin resistance cassette, pilA1 with chloramphenicol resistance cassette, and knh with kanamycin resistance cassette | 17 |
| KK03 ctrA::erm pilA1::cm hmw1CKk::kan(hmw1CKk) | Interruption of ctrA with erythromycin resistance cassette and pilA1 with chloramphenicol resistance cassette and reversion of interrupted hmw1CKk to wild type | This study |
| CCUG 11575 | Clinical isolate of A. aphrophilus | Culture collection of the University of Gothenburg |
| CCUG 11575 hmw1CAa::kan | Interruption of hmw1CAa with kanamycin resistance cassette | This study |
| CCUG 11575 hmw1CAa::kan(hmw1CAa) | Reversion of interrupted hmw1CAa to wild type | This study |
| CCUG 11575 emaA::kan | Interruption of emaA with kanamycin resistance cassette | This study |
| CCUG 11575 emaA::kan(emaA) | Reversion of the interrupted emaA to wild type | This study |
| E. coli BL21 pACYC HMW1CKk + pBAD | E. coli expressing HMW1CKk | This study |
| E. coli BL21 pACYC + pBAD Knh | E. coli expressing Knh | This study |
| E. coli BL21 pACYC HMW1CKk + pBAD Knh | E. coli expressing HMW1CKk and Knh | This study |