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. 2015 Jul 17;6:516. doi: 10.3389/fpls.2015.00516

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Copyright © 2015 Strzalka, Aggarwal, Krzeszowiec, Jakubowska, Sztatelman and Banas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of D-type cyclins subcellular localization and the formation of complexes with either PCNA1 or PCNA2. Confocal images of N. benthamiana leaf cells expressing transiently analyzed open reading frames. Split GFP complex formed between PCNA1_CtermGFP and (1) CycD1;1_NtermGFP, (2) CycD2;1_NtermGFP, (3) CycD3;1_NtermGFP, (4) CycD3;2_NtermGFP, (5) CycD3;3_NtermGFP, (6) CycD4;1_NtermGFP, (7) CycD4;2_NtermGFP, (8) CycD5;1_NtermGFP, (9) CycD6;1_NtermGFP, (10) CycD7;1_NtermGFP. Split GFP complex formed between PCNA2_CtermGFP and (11) CycD1;1_NtermGFP, (12) CycD2;1_NtermGFP, (13) CycD3;1_NtermGFP, (14) CycD3;2_NtermGFP, (15) CycD3;3_NtermGFP, (16) CycD4;1_NtermGFP, (17) CycD4;2_NtermGFP, (18) CycD5;1_NtermGFP, (19) CycD6;1_NtermGFP, (20) CycD7;1_NtermGFP. Split GFP complex formed between RFP150_CtermGFP and (21) CycD1;1_NtermGFP, (22) CycD2;1_NtermGFP, (23) CycD3;1_NtermGFP, (24) CycD3;2_NtermGFP, (25) CycD3;3_NtermGFP, (26) CycD4;1_NtermGFP, (27) CycD4;2_NtermGFP, (28) CycD5;1_NtermGFP, (29) CycD6;1_NtermGFP, (30) CycD7;1_NtermGFP. (31) CycD1;1_GFP, (32) CycD2;1_GFP, (33) CycD3;1_GFP, (34) CycD3;2_GFP, (35) CycD3;3_GFP, (36) CycD4;1_GFP, (37) CycD4;2_GFP, (38) CycD5;1_GFP, (39) CycD6;1_GFP, (40) CycD7;1_GFP. All the images are overlays of the bright field, autofluorescence of chlorophyll (red) and GFP fluorescence (green). The PCNA/CycD complexes are in the magenta frame. This result is representative of three independently repeated experiments.