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. 2015 Jul 17;6:516. doi: 10.3389/fpls.2015.00516

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Copyright © 2015 Strzalka, Aggarwal, Krzeszowiec, Jakubowska, Sztatelman and Banas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of interactions between Arabidopsis PCNA1/2 and D-type cyclins using the split-uiquitin Y2H system. The interactions were tested in the following combinations. (A) PCNA1 (bait)/D-type cyclins (prey), (B) PCNA2 (bait)/D-type cyclins (prey), and (C) D-type cyclins (bait)/PCNA1/2 (prey). The transformed yeast cells were plated on either an SC-Leu-Trp control solid medium or an SC-Leu-Trp-His selection solid medium supplemented with 10 mM 3-aminotriazol (3-AT). For the beta-galactosidase assay, the yeast transformants were grown on a nitrocellulose filter placed on the surface of a YPAD solid medium followed by incubation with X-gal. The results are representative of three independently repeated experiments.