Table 1.

Effect of the D73N mutation on the Ca²⁺ sensitivity and the rate of Ca²⁺ dissociation from reconstituted thin filaments in the absence and presence of cTnI pseudo-phosphorylation.

Parameter	cTn	cTn (with cTnIS22D/S23D)	cTnD73N	cTnD73N (with cTnIS22D/S23D)
pCa50	5.45±0.06	5.06±0.03†	4.90±0.05*	4.78±0.06‡≠
nH	1.45±0.5	1.6±0.1	1.68±0.08	2.2±0.8‡#≠
Ca2+ koff (s−1)	103±2	320±6†	341±16*	397±14‡#≠

All values are expressed as mean ± S.E. for at least three experiments per group.

^*cTn^D73N values significantly different from their respective cTn values;

^†cTn (with cTnI^S22D/S23D) values significantly different from their respective cTn values;

^‡cTn^D73N (with cTnI^S22D/S23D) values significantly different from their respective cTn (with cTnI^S22D/S23D) values;

^#cTn^D73N (with cTnI^S22D/S23D) values significantly different from their respective cTn^D73N values;

^≠cTn^D73N (with cTnI^S22D/S23D) values significantly different from their respective cTn values. The p-value < 0.05 was considered statistically significant.