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Concentrations of carbohydrates (A) and proteins (B) in whole biofilms of S. acidocaldarius and different biofilm fractions after EPS extraction with shaking, CER, crown ether (Cr.Eth.), EDTA, or NaOH. EPS were obtained from biofilms recovered from Brock medium plate cultures after incubation for 4 days at 78°C (n = 3). TEM, total extracellular material.
