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Total cell counts and plate counts of S. acidocaldarius after EPS extraction using shaking, CER, NaOH, crown ether (Cr.Eth.), and NaOH. Measurements were performed using suspensions of intact biofilm and treated cells in phosphate buffer (pH 7.0). Total cell counts were determined via counting of DAPI-stained cells. Colonies formed on Brock medium plates were counted after incubation for 4 days at 78°C (n = 3).
