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EPS proteins of S. acidocaldarius biofilms separated by 2 DE and visualized by silver staining. EPS were isolated from biofilms collected from Brock medium plates after 4 days of incubation at 78°C. A total of 200 μg EPS protein were applied for IEF (non-linear IPG strips). Molecular mass marker: PageRuler Prestained Protein Ladder (Fermentas).
