Figure 5.
The treatment with nicotine induces Akt-S6 pathway activation in human semimature DCs. (a–c) Human PBMC-derived semimature DCs were stimulated with nicotine (10−7 mol/L) for 15 min and the phosphorylation of Akt and ribosomal protein S6 was determined by flow cytometry (a), Western blotting (b), and confocal microscope analyses (c), respectively. One representative from 3 independent experiments is shown. Numbers in histogram indicated mean fluorescence intensity (MFI) of test samples. Data were given as mean ± SEM, Student t-test, ∗∗ P < 0.01. Ni: nicotine.