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. Author manuscript; available in PMC: 2015 Aug 27.
Published in final edited form as: Clin Exp Pharmacol Physiol. 2011 Aug;38(8):501–509. doi: 10.1111/j.1440-1681.2011.05537.x

Fig. 6.

Fig. 6

Effects of leukaemia inhibitory factor (LIF) on the proliferation of L6 cells are abolished by c-Myc knockdown. (a) Western blot of c-Myc protein expression 12 h after LIF treatment after transfection with either scrambled or c-Myc short interference (si) RNA and graphs showing band density normalized against α-tubulin. Quantitative analysis (arbitrary units) was determined using ImageJ software version 1.42q (National Institutes of Health, Bethesda, MD, USA; available from http://rsb.info.nih.gov/ij/, accessed 11 June 2009). Data are the mean ± SEM of three separate experiments. **P < 0.01 compared with scrambled siRNA. (b) Changes in L6 cell numbers after c-Myc siRNA transfection. Data were collected at 24 and 48 h after serum-free medium (DMEM) was replaced with DMEM + LIF 1 ng/mL. Data are the mean ± SEM of three separate experiments. †P < 0.01 compared with DMEM + LIF (24 h); **P < 0.01 compared with no c-Myc siRNA transfection (independent Student’s t-test).