Table 1.
Primer sequences used for cloning of cylM and its mutants
| Primer name | Primer sequence (5′-3′) |
|---|---|
| CylM_EcoRI_Duet_FP | AAAAA GAATTCG GAAGATA ATCTGATTAA T |
| CylM_NotI_Duet_RP | AAAAA GCGGCCGC TTACAGT TCAAACAGCA G |
| CylM_D252A_QC_FP | AGGGT GCA AGCCAT AGCCGTGGTAAAACCGTT AGC |
| CylM_D252A_QC_RP | ATGGCT TGC ACCCT GGC TTTCGCTAAT GCTATTCAGT |
| CylM_H254A_QC_FP | GATAGC GCT AGCCGT GGT AAAACCGTT AGCACCCTG |
| CylM_H254A_QC_RP | ACGGCT AGC GCTA TC ACCCTGGC TTTCGCTAAT G |
| CylM_D347A_QC_FP | GTTACC GCT CTGCAT TATGAAAACATCATTGCCCATGGC |
| CylM_D347A_QC_RP | AT GCAG AGC GGT AAC ATTAAAC AGAAAGGCAA TGCCAATCAG |
| CylM_H349A_QC_FP | CCGATCTG GCT TATGAAAA CATCATTGCCCATGGCGAATA |
| CylM_H349A_QC_RP | TTTTCATA AGC CAGATCGG T AACATTAAAC AGAAAGGCAA TGCCAAT |
| CylM_N352A_QC_FP | CATTATGAA GCC ATCATTGC CCATGGCGAATATCCG GTGATT |
| CylM_N352A_QC_RP | GCAATGAT GGC TTCATAATG CAGATCGGT AACATTAAAC AGAAAGGC |
| CylM_D364A_QC_FP | GTGATTATT GCT AATGAAACC TTTTTTCAGCAGAATATTCCGATTGAATTT |
| CylM_D364A_QC_RP | GGTTTC ATT AGC AATA ATCAC CGGAT ATTCGCCATG GGC |
| CylM_R506A_QC_FP | TGATTGTG GCC AATGTTAT TCGTCCGACCCAGCGTTA |
| CylM_R506A_QC_RP | A TAACATT GGC CACAATCA GA TTCTGCAGAT TATTATTAAT ATAGGCCAGA |
| CylM_T512A_QC_FP | GTCCG GCC CAG C GTTATGCAGATATGCTGGAA TTTAGC |
| CylM_T512A_QC_RP | CTG GGCCGGAC GAA TAACATTGCG CACAATCAGA |
| CylM_NdeI_FP | AAAAA CATATG GAAGATA ATCTGATTAA T |
| CylM625_KpnI_RP | AAAAA GGTACC TTA GTACGGGTTA TAAATATTCA G |