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. 2015 Jun 18;24(18):5115–5125. doi: 10.1093/hmg/ddv230

Figure 4.

Figure 4.

Analysis of hepatic ChREBP signaling in 70–90-week-old wild-type and AAV mice. (A) Hepatic G6P levels from wild-type (n = 26) and AAV (n = 20) mice. (B) Quantification of ChREBP mRNA by real-time RT–PCR, ChREBP protein levels by densitometric and western-blot analysis of ChREBP or β-actin. For quantitative RT–PCR, data represent mean ± SEM for wild-type (n = 24) and AAV (n = 22) mice, and for densitometric analysis, data represent mean ± SEM of four separate replicas of western blots. (C) Immunohistochemical analysis of hepatic ChREBP nuclear localization and quantification of nuclear ChREBP-translocated cells. Plates shown, from left to right, are at magnifications of ×100, ×400 and ×400. Data represent mean ± SEM for wild-type (n = 5) and AAV (n = 11) mice. (D) Quantification of mRNA for L-PK, ACC1, ElOVL6, FAS and SCD1 by real-time RT–PCR. Data represent mean ± SEM for wild-type (n = 24) and AAV (n = 22) mice. *P < 0.05 and **P < 0.005.