Table 2.
Primers
| cDNA primers for cloning of UBE2T into S91-IRES-NEO | |
| 5′ NotI | CATGCGGCCGCATGCAGAGAGCTTCA |
| 3′ BamH I | GTAGGATCCCTAAACATCAGGATG |
| cDNA primers | |
| 1F | AGTCAGAGGTCGCGCAGGCGCTG |
| 1R | CCCTCACAACGCAGCAA |
| 2F | TGCATCCCAGGCAGCTCTTA |
| 2R | TAGAAGGAACCACACACAGTTC |
| 3R | CATAAGGTGTGTTGGCTCCACCTA |
| 4R | CATCCAGACAAATCCTTCCAGCAG |
| 5R | TGGGTTCTGACATGAGCAGCTGAA |
| 6F | CAAGAATGCCAGACAGTGGACAGA |
| 6R | CTTGAGGAAGGCTGGCTTATTA |
| 7R | GGTAGGCAACTTAGATCACCTTGGCA |
| Genomic primers for the duplicationa | |
| ivs5F | CCTCTGCAACACATATCCTACC |
| ivs1R | CCTCTGTGCGTCTACATCTATTT |
| e7R | TCTATGCCTACTAGCTGACTGG |
| Genomic primers for the deletiona | |
| 1_7F | GCTTCTTTCCCGGTGGATTA |
| 1_7R | CCCAGACACACATTCAGGATAAA |
| ivs1R | AAACTCATGCTTCAGCCACACTGC |
| Primers for NMD detectiona | |
| Exon 1 | tgtaaaacgacggccagtAGTCAGAGGTCG CGCAGGCGCTGb |
| duplication-specific | gcctgggatgcActtttgtttctgc |
aAll primers shown as 5′ to 3′, F = forward, R = reverse.
bContains the M13 forward binding site (small letters).
cUnderlined A indicates the site of the junction in cDNA.