Figure 7.

FANCB regulates H3K9 methylation on sex chromosomes. (A–F, H, I, K and L) Immunostaining of chromosome spreads. Arrows: sex chromosomes. Scale bars: 10 µm. (G and J) Quantification of H3K9me2/3 signal intensity on sex chromosomes and autosome regions. Relative Mean Fluorescence Intensity (RMFI) was calculated as described in the Materials and Methods section. Mean and SEM for independent mice are shown. Numbers of analyzed nuclei are indicated in the panels. Three independent mice were analyzed for both wild-type and Fancb mutant mice. *P < 0.05 by one-way ANOVA followed by Tukey's range test.