Figure 3.

Functional studies of the FANCM mutation. (A) Analysis of cellular MMC sensitivity. MEFs expressing Δ22-FANCM allele (Fancm^−/− + Δ22 FANCM) are more sensitive to MMC than the cell expressing the wt FANCM allele (Fancm^−/− + wt FANCM). Not transduced MEFs (MEF Fancm^−/−) and MEF Fancm^−/− expressing a FANCM with loss-of-function mutation p.S724X (Fancm ^−/− + stop FANCM) are used as controls (N = 3; error = standard deviation). (B) Chromosome fragility induced by DEB treatment. Fancm^−/− + Δ22 FANCM and Fancm^−/− + stop FANCM cells show higher chromosome fragility than Fancm^−/− + wt FANCM. Twenty metaphases were analysed for chromosome breaks. Results are represented as mean number of breaks per cells and the error bars are SEM.