Figure 1.

Purification of dual-tagged p55. (A) Purification scheme. See text for details. (B and C) Western blots of three forms of p55 probed with either (B) Anti-penta HIS antibody or (C) Strep-Tactin alkaline phosphatase (AP) conjugate. The amount of protein loaded per well in μg is indicated above each well of the blot. Lower panels of (B) and (C) are Coomassie stained immunoblots following autoradiography and quantitation, and emphasize proper sample loading, high specificity of each probe for its tag and for each tag for its resin. WT NHis-p55 dimers [H in (A) and NHIS in (B) and (C)]; WT NStrep-p55 dimers (S and NStrep); dual-tagged WT NHis-p55/NStrep-p55 dimers (H/S and dual).