Table 1.

Estimated dissociation and rate constants

	Kd(DNA) (nM)a	kcat (s−1)b	kcat (s−1)b Spiked with WT p55c
Isolated p140	N/A	0.02 ± 0.001	0.27 ± 0.01
p140 + WT/WT p55	24 ± 9	0.53 ± 0.02	0.56 ± 0.04
p140 + WT/G451E p55	63 ± 2	0.23 ± 0.01	0.37 ± 0.05d

K_d(DNA) and k_cat values were estimated as described under Materials and Methods.

^aValues reported are averages with standard deviations.

^bStandard error estimates were derived by linear regression analysis to calculate apparent k_cat values.

^cA 1 nMolar equivalent of WT p55 was added to the reaction at 6.5 min and k_cat values were estimated using linear regression post WT p55 addition. See Figure 3 and Materials and Methods.

^dWhen a 1 nMolar equivalent of G451E p55 heterodimer is added to the G451E p55 heterodimer reaction in place of WT p55 then the estimated k_cat post G451E p55 heterodimer addition is 0.2 ± 0.01, standard error estimate derived by linear regression analysis.