Table 5. Activity of protein tyrosine kinases listed using the radiometric assay method. [Y136]WT: PSNSPPYVCYKK. [F136]MT: PSNSPPFVCYKK.
| Kinases | WT(CPM) | MT(CPM) | Difference(WT-MT) | %CFC |
|---|---|---|---|---|
| ROS1 | 124,391 | 24,140 | 100,251 | 81 |
| HCK | 77,752 | 7,469 | 70,283 | 90 |
| ABL1 | 64,612 | 20,116 | 44,496 | 69 |
| ABL2 | 55,031 | 18,183 | 36,848 | 67 |
| JAK3 | 50,944 | 6,172 | 44,772 | 88 |
| LCK | 46,181 | 3,251 | 42,930 | 93 |
| TYR03 | 42,310 | 5,282 | 37,028 | 88 |
[Y136]WT: PSNSPPYVCYKK and [F136]MT: PSNSPPFVCYKK which include Tyrosine 136 and Phenylalanine 136, were used as substrate to identify protein tyrosine kinases. % CFC: % change from control.