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. 2015 Aug 27;5:13588. doi: 10.1038/srep13588

Figure 2. FABP4 reversed the promoting functions of leptin on fatty acid oxidation.

Figure 2

The DMEM was used as the control against leptin treatment and cells were incubated with 100 nM leptin for 24 h or without leptin treatment. (A). Cell viability was detected by CCK-8 kit after 100 nM leptin treatment for 0 h, 6 h, 12 h, 18 h and 24 h (n = 6). (B). The mRNA expression of adipogenesis genes FATP1 and FAT after transfection with control vector, pc-FABP4 and si-FABP4 (n = 6). (C). Protein expression of FATP after transfected with control vector, pc-FABP4 and si-FABP4 (n = 6). (D). Protein expression of FAT after transfection with control vector, pc-FABP4 and si-FABP4 (n = 6). (E). Protein expression of FAS after transfected with control vector, pc-FABP4 and si-FABP4 (n = 6). (F). Protein expression of ACC after transfected with control vector, pc-FABP4 and si-FABP4 (n = 6). (G). Protein expression of CPT-1 after transfected with control vector, pc-FABP4 and si-FABP4 (n = 6). (H). Protein expression of AOX1 after transfection with control vector, pc-FABP4 and si-FABP4 (n = 6). All the protein levels were detected by ELISA test; pc-FABP4: FABP4 overexpression vector; si-FABP4: FABP4 shRNA vector; control: pcDNA 3.1-vector; FATP1: Fatty acid transport protein1, FAS: fatty acid synthase, CPT-1: carnitine palmitoyl transferase-1, ACC: acetyl-CoA carboxylase, AOX1: acyl-coenzyme A oxidase; Values are means ± SD. vs. control group, *p < 0.05, #p < 0.05.