Table 1. Primers used for PCR.
| Gene | RT-PCR primers (5′-3′) | Tm (°C) | Accession No. |
|---|---|---|---|
| CPT-1 | Fa: CTTCCAAGGCAGAAGAGTGGG | 60.2 | NM_013495.2 |
| Rb: GAACCTTGGCTGCGGTAAGAC | |||
| PGC1-α | F: CATGGATGGCCTATTTGATGAC | 58.3 | NM_133249 |
| R: CACGGAGAGTTAAAGGAAGAGC | |||
| NRF-1 | F: GCACCTTTGGAGAATGTGGT | 56.5 | BC005410 |
| R: GGGTCATTTTGTCCACAGAGA | |||
| AOX1 | F: CTGGAGCCCGCTTTGTCTT | 56.6 | NM_001271898.1 |
| R: CCTCGCCTTTCTTGATGGA | |||
| Cyt C | F: AAATCTCCACGGTCTGTTCG | 56.6 | NM_007808.4 |
| R: TGCCCTTTCTCCCTTCTTC | |||
| COX2 | F: TGACAGTCCACCTACTTACAAT | 55.2 | AF378830.1 |
| R: CTCCACCAATGACCTGATAT | |||
| β-actin | F: ACTGCCGCATCCTCTTCCTC | 56.7 | AY550069 |
| R: CTCCTGCTTGCTGATCCACATC | |||
| TFAM | F: AGCAGGTTTACGAGGAAGCA | 59.31 | NM_204100.1 |
| R: TTGAAGCCACTTCGAGGTCT | |||
| FABP4 | F: ATGTGCGACCAGTTTGTG | 50.8 | NM_024406 |
| R: TTTGCCATCCCACTTCTG | |||
| FATP1 | F: CGGTGCTGTTACGAGTGA | 61.5 | NM_001039602 |
| R: CACGGCGTTGGAATACTT | |||
| FAT | F: CTGGGAAGGTTACTGCGATTT | 52.9 | DQ323177 |
| R: TTCACGGTCTTACTGGTCTGG | |||
| UCP2 | F: GTGGATGCCTACAGGACCAT | 60.1 | AF287144.1 |
| R: GAAGTGACAGGGGACGTTGT | |||
| ACC | F: CCATATGGACCTCTTCTTGT | 58.8 | AB004329.1 |
| R: AAGTCCTTTCGCCTTGCAGT |
Fa: Forward primer.
Rb: Reverse primer.