Figure 2. Human DMD hearts show altered Cx43 localization and expression.

(a) Representative immunofluorescence images of heart cryosections from non-DMD and DMD patient hearts with antibodies reactive to Cx43 (green) and WGA (red), to demarcate cell borders, as well as DAPI to stain nuclei (blue). (b) Quantification of intercalated disc Cx43 expression, represented as percent of total Cx43 localized to intercalated discs with results from DMD1 and DMD2 combined for DMD. (c) Representative cropped western blot images of non-DMD and DMD patient heart protein homogenates probed for Cx43, with GAPDH as a loading control. Arrows indicate np and p bands of Cx43. Images of full-length blots are available as Supplemental Figure 3. (d) Quantification of (c), represented as fold difference of combined DMD1 and DMD2 results, represented as DMD, compared to non-DMD. * = p < 0.05 compared to non-DMD Human.