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. 2015 Sep;88(3):524–533. doi: 10.1124/mol.115.099093

Fig. 2.

Fig. 2.

Effects of Cmpd1, resveratrol, and pterostilbene on Stat3 tyrosine and serine phosphorylation and Stat3 acetylation in human glioma cells. Immunoblots of pYStat3, pSer727Stat3, Stat3, acetylated Stat3 (aStat3), and β-actin from whole-cell lysate preparation from the human glioma U251MG cells harboring aberrantly active Stat3 untreated or treated for (A) 3 hours with 0–20 µM Cmpd1, (B) 0–24 hours with 15 µM Cmpd1, (C) 0–24 hours with 20 µM resveratrol (Res), (D) 0–24 hours with 20 µM pterostilbene (PTE), or (E and F) 0–24 hours with 15 µM Cmpd1. The positions of the proteins in the gel are labeled. Bands corresponding to the phospho-Stat3 or acetylated Stat3 protein levels in the gel were quantified by ImageQuant and calculated as a percentage of control (dimethyl sulfoxide) relative to the total proteins and β-actin levels. Control lane (0) represents whole-cell lysates prepared from 0.025% dimethyl sulfoxide–treated cells. Data are representative of three independent determinations.