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. 2015 Feb 6;308(9):H1051–H1064. doi: 10.1152/ajpheart.00499.2014

Fig. 4.

Fig. 4.

Small interfering (si)RNA results. A: BAECs. Western blots (28-kDa monomer and 56-kDa dimer bands, n = 4) show knockdown versus control at optimized 20 nM siRNA2 relative to the vector β-actin (42 kDa) control. Relevant lanes were cut from a larger gel. Densitometry showed 70.3% knockdown (P < 0.0005) using ImageJ (National Institutes of Health). B: rat aortic ECs (RAECs). Western blots (56-kDa dimer band) with rat-specific AQP1 siRNA are shown. Lane 1, control without siRNA; lane 2, control with Lipofectamine 2000 but no siRNA; lanes 3 and 4, duplicates of 20 pmol siRNA; lanes 5 and 6, duplicates of 10 pmol siRNA. For 10 pmol, the AQP1 dimer band dropped 67.9% (P = 0.001) and the combined monomer + dimer bands dropped 59.2% (P = 0.00084), normalized by the β-actin control.