FIG 5.

The EsPgrp1/2 transcript level is elevated predominantly in the crypts by 24 h postcolonization. (A and B) Visualization of V. fischeri by labeling of the 16S rRNA (green); (A′ and B′) expression of EsPgrp1/2 (red); (A″ and B″) visualization of host tissue by labeling the EsHsp90 transcript (blue), in which the label is overlaid with the two other labels; (A, A′, and A″) EsPgrp1/2 is expressed in the symbiotic (Sym.) crypt epithelium at 24 h postcolonization; (B, B′, and B″) in contrast, its expression is undetectable in the crypts of aposymbiotic (Apo.) animals at the same stage of development. d, duct; a, antechamber; b, bottleneck; c, crypt (as shown in Fig. 1).