FIG 2.

Thermostability and optimal reaction temperature of the combined mutants. (A) Thermostability curves for combined mutants. The purified wild-type and mutant enzymes were incubated at 50°C for different times. The activity without incubation was taken as 100%. (B) Optimal temperature for combined mutants. The activity was determined with 50 mM glycine-NaOH buffer (pH 10.5) containing 0.2% (wt/vol) PGA. The measurements were performed in three independent experiments. Error bars represent standard deviations.