TABLE 1.

Primers used for PCRs

Purpose or substitution	Primer direction	Sequence (5′–3′)
ep-PCR	Forward	ATGAATGGAGGCACAACCG
	Reverse	GTTGATAACACCTACTCCAG
N289H	Forward	TTTGTCAATACACCGCATAGTCATTTAAACTCT
	Reverse	AGAGTTTAAATGACTATGCGGTGTATTGACAAA
N227S	Forward	TATTTTAAAGACATTAGTGATACAGCGATTAAC
	Reverse	GTTAATCGCTGTATCACTAATGTCTTTAAAATA
K313R	Forward	TCAGCTACTCAAGCAAGATCATCTGTTGAACAA
	Reverse	TTGTTCAACAGATGATCTTGCTTGAGTAGCTGA
T178A	Forward	GAATCATTGGAAAGCGATGCTCGTCGG
	Reverse	CCGACGAGCATCGCTTTCCAATGATTC
S308L	Forward	AGTTACCAAGTCCAATTAGCTACTCAAGCAAAA
	Reverse	TTTTGCTTGAGTAGCTAATTGGACTTGGTAACT
R72S	Forward	GTTAAAAATCACAGTGGAAAAGCTC
	Reverse	GAGCTTTTCCACTGTGATTTTTAAC
N186D	Forward	CGGTCATACTGATGATGCATCATTAGC
	Reverse	GCTAATGATGCATCATCAGTATGAC
I211V	Forward	GTGTCCCGCTTGTTCGATACGCT
	Reverse	TCAGCGTATCGAACAAGCGGGACAC
A254T	Forward	AGGATCAGGACAAACTGACCCAACGAC
	Reverse	GTCGTTGGGTCAGTTTGTCCTGATCCT