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(A to D) Time series of B. megaterium carrying the pKMMBm2 plasmid. Time points are 120 (A), 160 (B), 190 (C), and 210 (D) min after gene induction. Producer cells developed red tips (arrows), indicating XylR-mCherry-plasmid complexes located at the old cell poles. (E) Microcolony of high and low producers. Occasionally, deeply red cells lacking GFP arose, which probably accumulated plasmid-XylR complexes (arrow); these cells stopped proliferating.
