FIG 6.

Localization of plasmids using FISH. (A) B. megaterium carrying pSSBm85, induced with xylose, showed an unequal distribution of plasmids. These preferentially remained at one cell pole. (B) In the absence of gene induction, pSSBm85 plasmid distribution is hardly affected. (C) The smaller precursor plasmid pBC16 was found clustered in several foci distributed all over the cell. (D) The same behavior was seen in B. subtilis carrying the pBC16 plasmid, resulting in distinct foci of plasmid accumulation.