FIG 2.

Production, display, and functionality of ARP1 in the different constructs generated in this study. (A and B) Production of ARP1 determined in culture cell extracts by Western blotting using monoclonal mouse anti-E-tag antibodies and HRP-labeled goat anti-mouse antibodies as primary and secondary antibodies, respectively. The signal was detected by chemiluminescence using the ECL Plus Western blotting detection system (GE Healthcare). The theoretical molecular masses of the different ARP1 constructs are 39.7 kDa (pAF900-ARP1), 59.7 kDa (pLB11), 64.9 kDa (pLB12), 70.2 kDa (pLB13), 104 kDa (pLB19), and 148.7 kDa (pLB20). (C and D) Flow cytometric analyses to study the display of ARP1 (C) and binding to rotavirus (D) in GG (UT) using different anchor domains. For the analysis of histogram C, samples were incubated with a monoclonal mouse anti-E-tag antibodies and FITC-conjugated goat anti-mouse antibodies. For the analysis of histogram D, samples were incubated with RRV particles, rabbit anti-RRV K230, and PE-conjugated donkey anti-rabbit antibodies.