FIG 4.

Complementation of GG (UT) with the genes welF and welE. (A) Production of ARP1 determined in culture cell extracts by Western blotting. (B and C) Flow cytometric analyses to study the display (B) and binding (C) of ARP1 in GG (EM233) and complemented strain. Strains GG (UT) and GG (UT) pAF900-ARP1 were included as negative and positive control, respectively. For the analysis of histogram B, samples were incubated with a monoclonal mouse anti-E-tag antibodies and FITC-conjugated anti-mouse antibodies. For the analysis of histogram C, samples were incubated with RRV particles, rabbit anti-RRV K230, and PE-conjugated donkey anti-rabbit antibodies.