FIG 1.

Dynamic analysis of E. tarda autoaggregation. (A) EseB-mediated E. tarda autoaggregation. Deletion of eseB abolished E. tarda autoaggregation, and expression of eseB in pJN105-eseB induced by 50 mM l-arabinose restored autoaggregation to wild-type levels. (B) Autoaggregation of wild-type (wt), ΔeseB, and ΔeseB/pJN105-eseB cells in DMEM at 25°C. The wild-type and the complementing strains began to autoaggregate at 20 hps. (C) Autoaggregation observed by SEM. Cell clumps that settled on coverslips were observed for the wild-type and ΔeseB/pJN105-eseB strains but not the ΔeseB strain. Bars, 4 μm.