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. 2015 Aug 7;81(17):5845–5854. doi: 10.1128/AEM.01699-15

FIG 1.

FIG 1

Development of three FRET probe-based real-time PCR genotyping assays. (A) Differentiation of five human-pathogenic Cryptosporidium species (C. hominis, C. parvum, C, meleagridis, C. canis, and C. felis) in clinical specimens by 18S-LC1-based melt curve analysis. (B) Differentiation of the most common Cryptosporidium species (C. andersoni) in water from the three most common Cryptosporidium species (C. parvum, C. hominis, and C. meleagridis) in humans by 18S-LC2-based melt curve analysis. (C) Differentiation of three major human-pathogenic Cryptosporidium species (C. hominis, C. parvum, and C. meleagridis) by hsp90-based melt curve analysis. The melt peaks for each Cryptosporidium species are labeled.