Differentiation of Cryptosporidium species/genotypes commonly found in source water (C. parvum, C. hominis, C. andersoni, C. ubiquitum, deer mouse genotype III [W1], muskrat genotype I [W7], skunk genotype [W13], C. meleagridis, and C. cuniculus) by using melt curve analysis in the three FRET probe-based real-time PCR assays. (A) 18S-LC1 assay; (B) 18S-LC2 assay; (C) hsp90 assay. The melt peaks for each Cryptosporidium species are labeled.