TABLE 2.

Comparison of relative sensitivities of three FRET probe-based real-time PCR assays using various Mg²⁺ concentrations

Assay	Specimen	Species	Mg2+ concn (mM)	CT	Tm (°C)
18S-LC1	19283	C. parvum	1.5	19.65	54.27
			3.0	18.80	57.29
			4.5	18.02	58.65
			6.0	17.61	59.67
	19310	C. hominis	1.5	24.36	62.39
			3.0	23.12	64.99
			4.5	22.54	66.22
			6.0	21.82	67.08
18S-LC2	19283	C. parvum	1.5	23.41	60.12
			3.0	19.41	62.81
			4.5	19.13	64.02
			6.0	18.96	64.71
	19310	C. hominis	1.5	29.52	60.16
			3.0	23.99	62.81
			4.5	23.54	64.02
			6.0	23.39	64.71
hsp90	19283	C. parvum	1.5	28.30	48.50
			3.0	21.21	51.36
			4.5	20.22	52.55
			6.0	19.62	53.55
	19310	C. hominis	1.5	28.51	59.17
			3.0	25.74	62.09
			4.5	24.94	63.25
			6.0	24.27	64.24