TABLE 3.
Differentiation of Cryptosporidium species/genotypes commonly found in source water by using three FRET probe-based real-time PCR assaysa
| Specimen | Species or genotype | 18S-LC1 assay |
18S-LC2 assay |
hsp90 assay |
|||
|---|---|---|---|---|---|---|---|
| CT | Tm (°C) | CT | Tm (°C) | CT | Tm (°C) | ||
| 19289 | C. parvum | 25.04 | 57.48 | 25.44 | 62.93 | 27.03 | 51.58 |
| 19293 | C. parvum | 23.01 | 57.22 | 23.81 | 62.92 | 25.55 | 51.52 |
| 19314 | C. hominis | 16.98 | 65.08 | 17.88 | 62.92 | 19.13 | 62.28 |
| 19318 | C. hominis | 21.33 | 65.09 | 21.23 | 62.93 | 22.66 | 62.28 |
| 14934 | C. andersoni | − | − | 29.83 | 57.77 | 37.14b | 51.54b |
| 14937 | C. andersoni | − | − | 34.08 | 57.77 | − | − |
| 11689 | C. ubiquitum | − | − | 27.57 | 55.27 | − | − |
| 12355 | C. ubiquitum | − | − | 35.42 | 56.28 | − | − |
| 12350 | W1 | 26.65 | 60.01 | 28.36 | 62.36 | 30.27 | 56.27 |
| 12357 | W1 | − | − | 35.76 | 62.92 | − | − |
| 11706 | W7 | − | − | 37.38 | 58.28 | − | − |
| 11715 | W7 | − | − | − | − | − | − |
| 12376 | W13 | 28.86 | 57.43 | 31.70 | 55.70 | − | − |
| 13160 | W13 | − | − | − | − | − | − |
| 19208 | C. meleagridis | 27.44 | 60.81 | 28.87 | 62.92 | 30.30 | 56.74 |
| 19213 | C. meleagridis | 23.46 | 60.78 | 24.30 | 62.92 | 25.35 | 56.69 |
| 26224 | C. cuniculus | 30.23 | 65.13 | 33.54 | 62.92 | 35.49 | 62.35 |
| 26226 | C. cuniculus | 30.73 | 64.81 | 34.49 | 62.89 | 37.59 | 62.35 |
a
−, negative in PCR amplification; W1, deer mouse genotype III; W7, muskrat genotype I; W13, skunk genotype.
b
C. andersoni specimen 14934 appears to have a low level of C. parvum coinfection.