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d-Malate accumulation from maleate in strains SponMu and SponMu-hbzG::dmlA. The transformation of 1 mM maleate was catalyzed by permeabilized cells of strains SponMu and SponMu-hbzG::dmlA. The reaction conditions and sample management are described in Materials and Methods. Triple replicates were applied for each reaction. Data are presented as means ± SDs. The concentration of d-malate was monitored by HPLC over a period of 19 h.
