TABLE 2.
NAT assays included in feasibility study
| NAT assay use and amplification/detection method | Extraction method | Assay no. |
|---|---|---|
| NAT assays used for (semi)quantitative evaluation | ||
| In-house RT-PCR A | QIAamp viral RNA minikit | |
| In-house RT-PCR B | DNeasy blood and tissue kit | |
| In-house RT-PCR C | QiaSymphony | |
| In-house RT-PCR D | Phenol-chloroform | |
| Intego Mycoplasma | InviMag universal kit/IG | 4 |
| Intego Mycoplasma | Chemagen RSMI | 11 |
| Intego Mycoplasma | QIAamp DNA blood minikit | 15 |
| Microsart AMP Mycoplasma | InviMag universal kit/IG | 3 |
| MycoSEQ Mycoplasma real-time PCR | PrepSEQ sample preparation kit | 5, 8, 9, 10 |
| MycoSEQ Mycoplasma real-time PCR | NucliSENS easyMAG | 7 |
| MycoTool Mycoplasma real-time PCR | MagNA Pure | 1 |
| PLEX-ID Mycoplasma detection assay | Bead-beating lysis | 2 |
| Venor GeM qEP | QIAamp DNA blood minikit | 15 |
| NAT assays used for qualitative evaluation | ||
| CytoInspect PCR/microarray | CytoInspect DNA extraction kit | 18, 21, 24 |
| In-house nested PCR E | QIAamp DNA minikit | |
| In-house RT-PCR F | Phenol-chloroform | |
| In-house RT-PCR G | Silica columns | |
| MilliPROBE | Target capture (rRNA) | 26 |
| MycoTool Mycoplasma | Manual, 2-propanol | 19 |
| Venor GeM Advance | MB DNA extraction kit | 20 |
| Venor GeM Advance | QIAamp DNA blood minikit | 25 |