TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description or relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| P. damselae subsp. piscicida | ||
| DI21 | Highly virulent isolate, harbors pPHDP70, pPHDP60, pPHDP10 | Seabream, Spain |
| DI21-Rif | DI21 derivative, spontaneous Rifr mutant | 9 |
| CS31 | DI21 with pSJR52 inserted in pPHDP70; irp1 disruptant | 9 |
| AR84 | CS31 cured of plasmid pPHDP70 | This study |
| Vibrio alginolyticus | ||
| TA15 | Isolate from carpet shell clams (Ruditapes decussatus) | 31 |
| AR13 | TA15 ΔpvsA, deficient in vibrioferrin siderophore production | This study |
| AR43 | AR13 transconjugant for pPHDP70::Km | This study |
| AR45 | AR13 transconjugant for pPHDP70::pSJR52 | This study |
| Aeromonas salmonicida RSP74.1 | Isolated from turbot (Scophthalmus maximus) | 51 |
| Vibrio anguillarum RV22 | Isolated from turbot (Scophthalmus maximus) | 52 |
| Escherichia coli | ||
| DH5α | Cloning strain | Laboratory stock |
| S17-1 λ pir | RP4-2(Km::Tn7, Tc::Mu-1) pro-82 λpir recA1 endA1 thiE1 hsdR17 creC510 | Laboratory stock |
| XL1-blue MR | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac | Stratagene |
| MG1655 | E. coli K-12, F− | 53 |
| BI533 | MG1655 Nalr | 54 |
| MC1061 | F− araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL hsdR2(rK− mK+) mcrA mcrB1 | 55 |
| Plasmids | ||
| pKD4 | Template for Kmr gene | 21 |
| pNidKan | Suicide vector, derived from pCVD442, Kmr | 19 |
| pSJR52 | 4,672-bp EcoRI-XbaI fragment including the 3′ end of irp2 and the 5′ end of irp1, cloned into pNidKan | 9 |
| pPHDP70 | 68-kb native plasmid of strain DI21, contains piscibactin gene cluster | This study |
| pPHDP70::Km | pPHDP70 with a Kmr gene from pKD4 inserted between orf19 and orf20 | This study |
| pPHDP70::pSJR52 | pPHDP70 with pSJR52 inserted: disrupts irp1 gene | 9 |
| pPHDP60 | Conjugative 68-kb native plasmid of strain DI21 | 14 |
| pPHDP60::Cm | pPHDP60 with a Cmr gene inserted into orf48 | 14 |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Rifr, rifampin resistance; Cmr, chloramphenicol resistance; Nalr, nalidixic acid resistance.