TABLE 2.
Oligonucleotides used in this study
| Purpose and oligonucleotide | Sequence | bp amplified |
|---|---|---|
| entD-orf21 | ||
| HPI-103 | ATTGCGACGCATGTTGTATG | 2,134 |
| vibD-3′ | CATGCCTCCAAGGTTATCAC | |
| parAB | ||
| pCW046-5′-2 | ATTGCGACGCATGTTGTATG | 1,359 |
| pCW046-3′-1 | TATTCACGGTGATGACTAAC | |
| orf28 (putative carbonic anhydrase) | ||
| mig5-5′-1 | ACGTTTGTTTTAGCAACAGC | 459 |
| mig5-3′-1 | TGTCACCTAATGATGTGGTG | |
| orf48 (fimbrial usher) | ||
| pCW042-5′4 | CATTATGTCAGCATCTTCCG | 522 |
| usher-1 | TACTCGTGTAGTTATATCCG | |
| repA | ||
| RepA-F | GAAAGCTCAGATGCCATGCC | 2,407 |
| pCW044-5-1 | GAAAGCTCAGATGCCATGCC | |
| Amplification of pPHDP70 iterons | ||
| ITERON_F | GAAAGCTCAGATGCCATGCC | 1,429 |
| ITERON_R | GAAAGCTCAGATGCCATGCC | |
| Kmr marker amplification from pKD4 | ||
| Kan-1 | GCGGATCCGGCTTACATGGCGATAGCTA | 1,115 |
| Kan-2 | GCGGATCCCCCCGCGCTGGAGGATCATC | |
| Flanking DNA between orf19 and orf20 for Kmr marker insertion in pPHDP70 | ||
| HPmut1 | GCTCTAGACTATCTAACGCTTCGGGGAA | 3,314 |
| HPmut2 | GCGGATCCGCATTTCAGGACGATACACC | |
| HPmut3 | GCGGATCCGTTTACACATTGAGGACGAA | 3,314 |
| HPmut4 | GCGAATTCTGGCGCGGCATTTAAGGCCA | |
| Amplification of the Kmr marker and flanking pPHDP70 DNA | ||
| HP-Sac-A | GCGAGCTCCTATCTAACGCTTCGGGGAA | 7,771 |
| HP-Sac-B | GCGAGCTCTGGCGCGGCATTTAAGGCCA | |
| V. alginolyticus pvsA deletion | ||
| TA15-pvsAMut1 | GCGGGCCCCGTTGACGACCACAGTACGA | 1,088 |
| TA15-pvsAMut2 | GCGAATTCATGGGTCAGCGCACGTGCAC | |
| TA15-pvsAMut3 | GCGAATTCCGGATTAAAGTGACTATCAC | 1,015 |
| TA15-pvsAMut4 | GCTCTAGAAATACGCCCGTTGACCTTAT |