Table 2.

qRT-PCR primers used in the validation assays

Primer name	Sequence (5′–3′)	Amplicon length
Oct-FI	TGTTCCTGCAGTGCCCGAAA	109
Oct-F1	TTCTGGCGCCGGTTACAGAA
Sox2-F1	GAACCATCTCTGTGGTCTTG	316
Sox2-R1	GATTCTCGGCAGACTGATTC
Kif4-F1	CCGCTCCATTACCAAGAGCT	250
Kif4-R1	TGGTCAGTTCATCTGAGCGG
c-myc-F1	ACACATCAGCACAACTACGC	159
c-myc-R1	CCTCTTGACATTCTCCTCGGT
Nanog-F1	TTCTGCTGAGATGCCTCACA	322
Nanog-R1	CATTCTCTGGTTCTGGAACC
Lin28-F1	TGGGGTGTGTTTATTTGATGG	96
Lin28-R1	GGGAAGGGAAGAGTCAGCTT
SALL4-F1	GACTCACACTGGAGAGAAGC	170
SALL4-R1	TTCCGTCCGTACCTAACAGA
REX1-F1	CACAGTCCATCCTTACAGAGTT	160
REX1-R1	CGTTCAGTGCCTTCTCTATG
GAPDH-F1	GGGAAACTGTGGCGTGAT	299
GAPDH-R1	GAGTGGGTGTCGCTGTTGA