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. 2015 Sep 1;26(17):3001–3012. doi: 10.1091/mbc.E14-10-1427

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© 2015 Haase, Zimmermann, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Ectopic expression of TgADF-cMyc, PfADF1-cMyc, and the corresponding Lys mutants in TgADF cKO parasites. (A) Western blot analysis of the parasite lines grown with or without ATc for 48–50 h. Parasite protein lysates were probed with anti-HA to control for down-regulation of the inducible TgADF-HA copy, as well as anti-cMyc and anti-TgADF to control for ectopic protein expression. Anti-Act_239-253 was used as a loading control. (B–F) Immunofluorescence analysis of parasites grown with or without ATc demonstrates cytosolic localization of inducible TgADF-HA (A) and the complementing cMyc-tagged proteins (C–F). Scale bar, 5 μm (C–F).