FIGURE 2:

TgADF_K68A-cMyc–expressing parasites cannot restore TgADF cKO loss of function. (A–E) Plaque assays of plated human fibroblasts grown with each parasite treated with or without ATc for 8 d. White plaques represent characteristic areas where host cells have been lysed due to parasite propagation. A growth defect is seen in A (bottom right) for the TgADF cKO line, as expected. A similar phenotype is seen in D (bottom for both clones), where no plaques have been formed for TgADF_K68A-cMyc–expressing parasites grown in the presence of ATc. No obvious growth defect is detectable in other complementing lines.