FIGURE 4:

Parasite gliding motility is independent of Lys68/72-mediated actin filament turnover. Parasite lines were cultured with or without ATc for 68–72 h, harvested, and allowed to glide on poly-l-lysine–coated slides. Motility was captured by time-lapse videomicroscopy. The average from two independent experiments is shown (number of parasites, on average 500 per line and clone). Only the TgADF cKO parasite demonstrates a significant reduction in motile parasites (A) or markedly aberrant motility (C) compared with the four other complementing lines (A–D).