TABLE 1:
Set | Helical gliding motility (μm/s)a | ||||
---|---|---|---|---|---|
Set 1 | TgADF cKO | TgADF #3 | TgADF #5 | TgADFK68A #1 | TgADFK68A #6 |
−ATc | 0.82 ± 0.23 | 0.83 ± 0.20 | 0.81 ± 0.14 | 0.77 ± 0.23 | 0.75 ± 0.18 |
+ATc | N/Ab | 0.80 ± 0.18 | 0.82 ± 0.23 | 0.74 ± 0.16 | 0.71 ± 0.15 |
Set 2 | TgADF cKO | TgADF #3 | TgADF #5 | TgADFK68A #1 | TgADFK68A #6 |
−ATc | 0.75 ± 0.15 | 0.79 ± 0.18 | 0.82 ± 0.12 | 0.74 ± 0.13 | 0.74 ± 0.18 |
+ATc | N/Ab | 0.75 ± 0.18 | 0.79 ± 0.17 | 0.80 ± 0.20 | 0.74 ± 0.13 |
Set 1 | PfADF1 #4 | PfADF1 #5 | PfADF1K72A #2 | PfADF1K72A #3 | |
−ATc | 0.83 ± 0.38 | 0.84 ± 0.29 | 0.79 ± 0.17 | 0.83 ± 0.30 | |
+ATc | 0.81 ± 0.19 | 0.81 ± 0.25 | 0.80 ± 0.15 | 0.84 ± 0.23 | |
Set 2 | PfADF1 #4 | PfADF1 #5 | PfADF1K72A #2 | PfADF1K72A #3 | |
−ATc | 0.79 ± 0.29 | 0.68 ± 0.16 | 0.77 ± 0.22 | 0.74 ± 0.16 | |
+ATc | 0.78 ± 0.26 | 0.71 ± 0.20 | 0.76 ± 0.17 | 0.73 ± 0.18 |
aMean speed ± SD measured over 60 s from n = 20 parasite trails per line, clone, and condition (± ATc).
bHelical gliding not observed for ATc-treated TgADF cKO parasites.