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. 2015 Sep 1;26(17):3061–3070. doi: 10.1091/mbc.E14-11-1547

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© 2015 Pei, Schnettger, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — The IFN-γ–dependent enlargement of the endocytic compartment in macrophages requires Rab20. (A) Distribution of EEA-1 in macrophages expressing scrambled shRNA (top) or Rab20 shRNA (bottom) with or without IFN-γ treatment. RAW264.7 macrophages expressing scrambled shRNA or Rab20 shRNA were treated with or without IFN-γ for 16–20 h and fixed for immunofluorescence. Scale bar, 10 μm. (B) Quantitative analysis of the area of EEA-1–positive structures in macrophages expressing scrambled shRNA or Rab20 shRNA with or without IFN-γ treatment. For each group, at least 20 cells were analyzed. Data show mean ± SD of three independent experiments. The p values were calculated using Student's two-tailed t test. *p ≤ 0.05. (C) Distribution of endogenous LAMP-2 in macrophages expressing scrambled shRNA (top) or Rab20 shRNA (bottom) with or without IFN-γ treatment. RAW264.7 macrophages expressing scrambled shRNA or Rab20 shRNA were stimulated with IFN-γ or left untreated for 16–20 h and fixed for immunofluorescence. Scale bar, 10 μm. (D) Quantitative analysis of the area of LAMP-2–positive structures in macrophages expressing scrambled shRNA or Rab20 shRNA with or without IFN-γ treatment. For each group, at least 20 cells per experiment were analyzed. Data show mean ± SD of three independent experiments. The p values were calculated using Student's two-tailed t test. *p ≤ 0.05.