TABLE 1:
Estimated copy number for all major CCV proteins.
| Protein/gene name | Copy number (from AP-1 ks) | Copy number (from epsinR ks) |
|---|---|---|
| Clathrin | ||
| Clathrin heavy chain/CHC17/CLTC | 180 | 180 |
| CHC22/CLTCL1 | 4.4 | 4.0 |
| Clathrin light chain A/CLTA | 97.2 | 97.3 |
| Clathrin light chain B/CLTB | 43.1 | 42.3 |
| AP-1 | ||
| AP-1 γ/AP1G1 | 60.7 | 47.8 |
| AP-1 β/AP1B1 | 62 | 50.7 |
| AP-1 μ1A/AP1M1 | 48.9 | 38.7 |
| AP-1 σ1A/AP1S1 | 29.3 | 23.1 |
| AP-1 σ1B/AP1S2 | 3.5 | 2.7 |
| Other peripheral membrane proteins | ||
| EpsinR/CLINT1 | 74.7 | 73.4 |
| ARF1 | 25.7 | 20.8 |
| CVAK104/SCYL2 | 13.1 | 13.0 |
| Rab4A | 11.9 | 9.2 |
| OCRL | 11.6 | 11.4 |
| Rab6A | 8.1 | 4.0 |
| PICALM | 7.6 | 8.2 |
| VPS45 | 6.7 | 4.8 |
| STAMBPL1 | 6.4 | 6.1 |
| PIK3C2A | 5.7 | 6.5 |
| Auxilin 2/GAK | 5.5 | 4.2 |
| Hip1R | 5.4 | 5.3 |
| DNAJC6 | 4.4 | 3.9 |
| Gadkin/C4orf16 | 3.4 | 2.4 |
| CLLD6/SPRYD7 | 3.3 | 2.5 |
| Dynamin 2/DNM2 | 2.6 | 2.9 |
| FAM84B | 2.3 | 1.9 |
| TRIM47 | 2.0 | 1.8 |
| Aftiphilin/AFTPH | 1.7 | 0.8 |
| Rab4B | 1.6 | 1.1 |
| GGA2 | 1.5 | 1.2 |
| γ-Synergin/AP1GBP1 | 1.4 | 0.7 |
| PI4K2B | 1.4 | 0.6 |
| p200/HEATR5B | 1.3 | 0.7 |
| LDLRAP1/ARH | 1.2 | 0.8 |
| AGFG1/Hrb | 1.0 | 0.8 |
| RABGEF1 | 0.7 | 0.6 |
| Kif13A | 0.6 | 0.4 |
| Integral membrane proteins | ||
| Cation-dependent M6P receptor/M6PR | 44.7 | 42.1 |
| Transferrin receptor/TFRC | 31.8 | 23.8 |
| VAMP2 (R-SNARE) | 23.4 | 15.6 |
| Cation-independent M6P receptor/IGF2R | 16.2 | 13.3 |
| VAMP8 (R-SNARE) | 11.2 | 10.0 |
| Syntaxin 10/STX10 (Qc-SNARE) | 11.0 | 6.7 |
| VAMP3 (R-SNARE) | 10.3 | 7.9 |
| Vti1b (Qb-SNARE) | 8.2 | 6.5 |
| Syntaxin 6/STX6 (Qc-SNARE) | 6.3 | 3.8 |
| Syntaxin 8/STX8 (Qc-SNARE) | 5.4 | 3.6 |
| SNAP29 (Qbc-SNARE) | 4.9 | 5.1 |
| Vti1a (Qb-SNARE) | 4.7 | 3.1 |
| YIPF6 | 4.0 | 2.7 |
| Syntaxin 16/STX16 (Qa-SNARE) | 3.7 | 2.6 |
| IRAP/LNPEP | 3.5 | 2.5 |
| Syntaxin 12/STX12 (Qa-SNARE) | 3.2 | 2.6 |
| TSPAN13 | 3.2 | 2.2 |
| Carboxypeptidase D/CPD | 2.9 | 1.9 |
| Sortilin/SORT1 | 2.2 | 1.8 |
| VAMP4 (R-SNARE) | 2.1 | 1.3 |
| Lumenal proteins/hydrolases | ||
| Cathepsin Z/CTSZ | 10.9 | 9.2 |
| N-acetylglucosamine-6-sulfatase/GNS | 5.6 | 5.3 |
| DNASE2 | 2.6 | 2.4 |
| Prosaposin/PSAP | 2.5 | 2.8 |
| Hexosaminidase B/HEXB | 1.7 | 2.5 |
| Palmitoyl-protein thioesterase 1/PPT1 | 0.8 | 0.6 |
| Hexosaminidase A/HEXA | 0.6 | 1.3 |
| Granulin/GRN | 0.6 | 0.9 |
| Phospholipase B domain 2/PLBD2 | 0.5 | 0.5 |
The numbers were deduced from our fractionation profiling data (Borner et al., 2014), our earlier AP-1 knocksideways (ks) data (Hirst et al., 2012), and the epsinR ks data from the present study.