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. 2015 Sep 1;26(17):3104–3116. doi: 10.1091/mbc.E15-06-0330

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© 2015 Stepanyants et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Drp1 preferentially remodels membranes containing a high spatial density of fluid-phase CL. (A–D) Stimulated GTPase activity of Drp1 WT (0.5 μM final) preassembled on liposomes of defined lipid composition (150 μM total lipid) containing varying mole fractions (mol%) of native CL plotted as specific activity (min⁻¹) ± SD (n = 3). (E) Confocal fluorescence images of surface-immobilized Rh-DOPE–labeled GUVs containing 10 mol% native CL (red; left) in the presence of BODIPY-FL-labeled Drp1 WT (0.5 μM protein final; green; middle). Right, merged images. (F) Confocal fluorescence images of GUVs phase-separated into raft-phase, liquid-ordered (l_o, NBD-DHPE–labeled, green), and fluid-phase, liquid-disordered (l_d, Rh-DOPE–labeled, red) membrane regions before (left) and after (right) addition of unlabeled Drp1 WT (0.5 μM final). Only merged images are shown. Arrow points to Drp1-generated membrane tubules originated from Rh-DOPE–labeled, fluid-phase membrane regions. (G) Same as F, but containing unlabeled, dark, raft-phase l_o regions before (left) and after (right) addition of BODIPY-FL–labeled Drp1 WT. Arrow points to Drp1-decorated membrane tubules originating from fluid-phase membrane regions. Only merged images are shown. Scale bar, 5 μm.