FIGURE 5:

Drp1 induces apparent GTP- and B-insert–dependent CL phase transition. (A) NBD emission intensity change in liposomes containing either 25 mol% native CL or TMCL in the presence of DOPC and DOPE (50 μM total lipid) upon addition of Drp1 WT (0.5 μM final). Relative extent of lamellar-to-H_II membrane phase transition induced by Ca²⁺ ions (5 mM CaCl₂ final; Supplemental Figure S2A) is shown for comparison. (B) NBD emission intensity change upon addition of Drp1 WT (0.5 μM final) to 25 mol% native CL-containing liposomes (50 μM total lipid) in the constant presence of various nucleotides (nucl; 1 mM final). (C) NBD emission intensity increase upon addition of various nucleotides (1 mM final) to Drp1 WT (0.5 μM final) preassembled on 25 mol% native CL-containing liposomes (50 μM total lipid) before nucleotide addition. (D, E) Comparison of the extent of NBD emission intensity increase observed for Drp1 WT on 25 mol% native CL-containing liposomes (Lipo) in the constant presence of nucleotides as in B with that of GTPase domain mutants (D) or B-insert mutants (E).