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. 2015 May 20;7(8):1004–1017. doi: 10.15252/emmm.201405010

Figure 2.

Figure 2

Reduced TNFR1 protein levels in S mice and genetic linkage study of this trait

  1. TNFR1 protein levels in serum and tissues of different mouse groups (n = 4–13). TNFR1 protein levels were measured by ELISA and compared to B, the levels of which were set as 100% for each tissue. From left to right: B mice (black), TNFR1+/- mice (grey), (BxS)F1 mice (grey), S mice (grey); B.S-chr6-BB (black), B.S-chr6-SS (green) and B.S-chr6-BS (grey).
  2. TNFR1 expression in splenic neutrophils of wild-type, TNFR1 KO and SPRET mice determined by FACS. Neutrophils were defined by flow cytometry (FACS) as SSChighCD11b+Ly6GhighLy6Cmid and TNFR1+ cells from different strains were plotted in a histogram. In the insert, the median of TNFR1-PE signals were plotted and this shows that SPRET mice have an intermediate TNFR1 expression compared to WT mice.
  3. TNFR1 mRNA levels measured by qPCR in organs of B (n = 3) and S (n = 4). Affymetrix microarray data from hepatocytes (Affy Mo Gene 1.0 ST Array).
  4. Backcross experiments in mice to determine genetic linkage between TNF resistance (left) and TNFR1 liver protein levels (right). QTL mapping of lethal response (n = 178) was based on the data previously obtained and discussed by Staelens et al (2002), but the data were re-analysed in the current study using R/qtl software. TNFR1 protein level in the liver of N2 mice (n = 214). For both TNF resistance and TNFR1 levels, the LOD scores show a QTL on chr2 (TNF resistance 15 cM, LOD = 2.25 and TNFR1 levels 5 cM, LOD = 1.31) and chr6 (TNF resistance 46 cM, LOD = 3.11 and TNFR1 levels 70 cM, LOD = 4.15). Horizontal lines in the figures represent LOD values that are suggestive (dots), significant (stripes) and highly significant (full line).

Data information: Data are presented as mean ± SE. Student’s t-test using unpaired comparisons and two-tailed analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.